Anti-SFTPC Polyclonal Antibody is a Rabbit antibody targeting SFTPC. Anti-SFTPC Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Cow,Rabbit,Sheep)

1. Cell: NCI-H460
Concentration:1:100
Host/Isotype: Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: TMAB-01682) on NCI-H460 (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit Igg (H+L) secondary antibody.
2. Blank control (black line): A549. Primary Antibody (green line): Rabbit Anti-SFTPC antibody (TMAB-01682)-PE
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Negative control (white blue line): PBS
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human SFTPC

Human