Anti-S100B Antibody (2F740) is a Mouse antibody targeting S100B. Anti-S100B Antibody (2F740) can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

2F740

Human,Mouse,Rat

1. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. 25 μg total protein per Lane of various lysates probed with S100B monoclonal antibody, unconjugated (TMAB-01645) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
12. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
13. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
14. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
15. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
16. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
18. The A375 (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Mouse Anti-S100B antibody (TMAB-01645): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488: 1 μg/test. Blank control (black): PBS.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Monoclonal

Recombinant Protein: human S100B protein

Human

S100B

Protein S100-B

Cell differentiation,Cell Differentiation,s100 beta,Astrocyte

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.