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Anti-S-tag Polyclonal Antibody 是一种 Rabbit 抗体,靶向 S-tag。Anti-S-tag Polyclonal Antibody 可用于 WB,ELISA。
Anti-S-tag Polyclonal Antibody 是一种 Rabbit 抗体,靶向 S-tag。Anti-S-tag Polyclonal Antibody 可用于 WB,ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 960 | 5日内发货 | |
| 500 μL | ¥ 3,980 | 5日内发货 |
| 产品描述 | Anti-S-tag Polyclonal Antibody is a Rabbit antibody targeting S-tag. Anti-S-tag Polyclonal Antibody can be used in WB,ELISA. |
| Ig Type | IgG |
| 反应种属 | Species independent |
| 应用 | WBELISA |
| 推荐剂量 | WB: 1:1000-5000; ELISA: 1:1000-5000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. The amino acid sequence of the S-tag is: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser. It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to[citation needed]. Moreover, the peptide alone is thought not to fold into a distinct structure. On DNA-level the S-tag can be attached to the N- or C-terminus of any protein. After gene expression, such a tagged protein can be detected by commercially available antibodies. |
| 免疫原 | KLH conjugated synthetic peptide: S-tag |
| 蛋白名称 | S tag |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | store at low temperature | -20°C for 1 year |