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顶部生物活性储存和溶解信息计算器

Anti-RNF20 Antibody (1Z606)

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Anti-RNF20 Antibody (1Z606)
产品编号 TMAC-03615
别名 RNF 20, RING-type E3 ubiquitin transferase BRE1A, RING finger protein 20, hBRE1, EC 2.3.2.27, E3 ubiquitin-protein ligase BRE1A, BRE1-A, BRE1A

Anti-RNF20 Antibody (1Z606) 是一种 Rabbit 抗体，靶向 RNF20。Anti-RNF20 Antibody (1Z606) 可用于 WB,IP,FCM。

Anti-RNF20 Antibody (1Z606)

Anti-RNF20 Antibody (1Z606)

Rating icon 还可以
Anti-RNF20 Antibody (1Z606)
产品编号 TMAC-03615 别名 RNF 20, RING-type E3 ubiquitin transferase BRE1A, RING finger protein 20, hBRE1, EC 2.3.2.27, E3 ubiquitin-protein ligase BRE1A, BRE1-A, BRE1A

Anti-RNF20 Antibody (1Z606) 是一种 Rabbit 抗体，靶向 RNF20。Anti-RNF20 Antibody (1Z606) 可用于 WB,IP,FCM。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-RNF20 Antibody (1Z606) is a Rabbit antibody targeting RNF20. Anti-RNF20 Antibody (1Z606) can be used in WB,IP,FCM.
别名RNF 20, RING-type E3 ubiquitin transferase BRE1A, RING finger protein 20, hBRE1, EC 2.3.2.27, E3 ubiquitin-protein ligase BRE1A, BRE1-A, BRE1A
Ig Type
IgG
交叉反应
Human,Mouse
验证活性
1. Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: MCF-7, Lane 3: Jurkat.
2. Flow cytometric analysis of K562 cells with RNF20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
IP
FCM
推荐剂量
WB: 1:1000; ICC: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF20 (ring finger protein 20), also known as BRE1, BRE1A or hBRE1, is a 975 amino acid nuclear protein that belongs to the BRE1 family. As a component of the RNF20/40 complex, RNF20 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B. RNF20 is required for transcriptional activation of Hox genes and is most likely recruited by p53 to the MDM2 promoter, thereby acting as a transcriptional co-activator. RNF20 contains one zinc finger domain and exists as a homodimer.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q5VTR2
化学信息
分子量Theoretical: 114 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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