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Anti-RheB Antibody (8S39)
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产品编号 TMAC-03596
别名 RHEB2, Ras homolog enriched in brain
Anti-RheB Antibody (8S39) 是一种 Rabbit 抗体，靶向 RheB。Anti-RheB Antibody (8S39) 可用于 WB,ICC,IF,IHC,FCM。
Anti-RheB Antibody (8S39)
Anti-RheB Antibody (8S39)
还可以产品编号 TMAC-03596 别名 RHEB2, Ras homolog enriched in brain
Anti-RheB Antibody (8S39) 是一种 Rabbit 抗体，靶向 RheB。Anti-RheB Antibody (8S39) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-RheB Antibody (8S39) is a Rabbit antibody targeting RheB. Anti-RheB Antibody (8S39) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | RHEB2, Ras homolog enriched in brain |
| Ig Type | IgG |
| 克隆号 | 8S39 |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Western blot analysis of RHEB on mouse placenta tissue lysate using anti-RHEB antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RHEB antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RHEB antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RHEB antibody. Counter stained with hematoxylin. 5. ICC staining RHEB in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining RHEB in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of A431 cells with RHEB antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | H-, K- and N-Ras represent the prototype members of a family of small G proteins which are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound rather than the inactive GDP-bound state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 or 87 mutants of R-Ras (analogous to positions 12 and 61 in H-Ras) have been shown to be capable of activating oncogenic function. Ras p21 in its active GTP binding state binds to Raf-1, resulting in activation of the MAP kinase signaling cascade. An additional member of the Ras family, Rheb (Ras-related GTP-binding protein), also interacts with Raf-1. This interaction is potentiated by growth factors and agents that increase cAMP levels. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: human RHEB aa 1-250 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 20 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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