Anti-RASA1 Antibody (1P612) is a Rabbit antibody targeting RASA1. Anti-RASA1 Antibody (1P612) can be used in FCM, IF, IHC-Fr, IHC-P, WB.

IgG

1P612

Human,Rat,Mouse

1. 25 ug total protein per lane of various lysates (see on figure) probed with RASA1 monoclonal antibody, unconjugated (TMAB-12103) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The U-87MG (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-RASA1 antibody (TMAB-12103, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RASA1 Monoclonal Antibody, Unconjugated (TMAB-12103) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

FCM=1:50-100; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

A synthesized peptide: human RASA1

Human

RASA1

Ras GTPase-activating protein 1

Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.