Anti-RAN Antibody (8O734)

Name: Anti-RAN Antibody (8O734)
Brand: TargetMol
SKU: TMAH-01032
Rating: 4.5 (1 reviews)

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产品编号 TMAH-01032

Anti-RAN Antibody (8O734) 是一种抗体，靶向 RAN。Anti-RAN Antibody (8O734) 可用于 ELISA, IHC, IF, FC。

Anti-RAN Antibody (8O734)

还可以

产品编号 TMAH-01032

Anti-RAN Antibody (8O734) 是一种抗体，靶向 RAN。Anti-RAN Antibody (8O734) 可用于 ELISA, IHC, IF, FC。

规格	价格	库存	数量
50 μL	¥ 1,310	5日内发货
100 μL	¥ 2,195	5日内发货

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产品描述	Anti-RAN Antibody (8O734) is an antibody targeting RAN. Anti-RAN Antibody (8O734) can be used in ELISA, IHC, IF, FC.
Ig Type	Rabbit IgG
克隆号	8O734
交叉反应	Human
验证活性	1. IHC image of TMAH-01032 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.78% DAB. 2. Immunofluorescence staining of Hela with TMAH-01032 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 527-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 3. Overlay Peak curve showing Hela cells stained with TMAH-01032 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/110^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
应用	ELISA, IHC, IF, FC
推荐剂量	IHC:1:50-1:200; IF:1:50-1:200; FC:1:50-1:200.
抗体种类	Monoclonal
亚细胞定位	Nucleus. Nucleus envelope. Cytoplasm, cytosol. Cytoplasm. Melanosome.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景	GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.