Anti-RAGE Polyclonal Antibody is a Rabbit antibody targeting RAGE. Anti-RAGE Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RAGE) Polyclonal Antibody, Unconjugated (TMAB-01604) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Cell: NIH/3T3
Concentration:1:100
Host/Isotype: Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: TMAB-01604) on NIH/3T3 (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit Igg (H+L) secondary antibody.
3. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-RAGE antibody (TMAB-01604)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Adrenal gland (Mouse) Lysate at 40 μg
Lane 3: Kidney (Rat) Lysate at 40 μg
Lane 4: Adrenal gland (Rat) Lysate at 40 μg
Primary: Anti-RAGE (TMAB-01604) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 58/50 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: rat AGER

Rat

Diabetes associated,Inflammatory mediators,Amyloid,Alzheimer's disease,Visual system