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Anti-PSMB8 Antibody (3I410)
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产品编号 TMAC-03441
别名 Y2, RING10, Really interesting new gene 10 protein, PSMB8, PSMB5i, Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome component C13, Multicatalytic endopeptidase complex subunit C13, Macropain subunit C13, Low molecular mass protein 7, LMP7
Anti-PSMB8 Antibody (3I410) 是一种 Rabbit 抗体，靶向 PSMB8。Anti-PSMB8 Antibody (3I410) 可用于 WB,ICC,IF,IHC,FCM。
Anti-PSMB8 Antibody (3I410)
Anti-PSMB8 Antibody (3I410)
还可以产品编号 TMAC-03441 别名 Y2, RING10, Really interesting new gene 10 protein, PSMB8, PSMB5i, Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome component C13, Multicatalytic endopeptidase complex subunit C13, Macropain subunit C13, Low molecular mass protein 7, LMP7
Anti-PSMB8 Antibody (3I410) 是一种 Rabbit 抗体，靶向 PSMB8。Anti-PSMB8 Antibody (3I410) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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| 产品描述 | Anti-PSMB8 Antibody (3I410) is a Rabbit antibody targeting PSMB8. Anti-PSMB8 Antibody (3I410) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | Y2, RING10, Really interesting new gene 10 protein, PSMB8, PSMB5i, Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome component C13, Multicatalytic endopeptidase complex subunit C13, Macropain subunit C13, Low molecular mass protein 7, LMP7 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Proteasome 20S LMP7 on U937 (1) and A431 (2) cell lysate using anti-Proteasome 20S LMP7 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Proteasome 20S LMP7 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Proteasome 20S LMP7 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Proteasome 20S LMP7 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Proteasome 20S LMP7 antibody. Counter stained with hematoxylin. 6. ICC staining Proteasome 20S LMP7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Proteasome 20S LMP7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Daudi cells with Proteasome 20S LMP7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The eukaryotic multi-catalytic proteinase complex, otherwise known as the proteasome, is present in both the nucleus and cytoplasm of cells and contains at least 15 nonidentical subunits, which form a highly ordered ring-shaped structure. The proteasome is involved in an ATP/Ubiquitin-dependent proteolytic pathway and expresses at least five distinct proteolytic activities, including the cleavage of peptides after branched-chain amino acids or bulky hydrophobic amino acids. Two components of the proteasome are the low molecular mass proteins LMP2 and LMP7, which are thought to connect the proteasome to the MHC class-I antigen-processing pathway. Upon stimulation with IFN-γ, LMP2 and LMP7 displace housekeeping subunits in the proteasome and activate cytotoxic T cells (CTLs). LMP2 and LMP7 are produced as precursor proteins, which are processed to subunits that have the ability to complex with the proteasome. LMP2 is expressed as two alternatively spliced forms, LMP2.l and LMP2.s, in lymphoblastoid cell lines and in fibroblasts after IFN-γ stimulation. LMP7 is also expressed as two forms, LMP7A and LMP7B, also designated LMP7-E1 and E2, in several tissues. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 25 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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