Anti-PKM Antibody (4D256)

Name: Anti-PKM Antibody (4D256)
Brand: TargetMol
SKU: TMAH-00983
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00983

别名 THBP1, TCB, pyruvate kinase, muscle, PKM2, PK3, OIP3, HEL-S-30, CTHBP

Anti-PKM Antibody (4D256) 是一种 Mouse 抗体，靶向 PKM。Anti-PKM Antibody (4D256) 可用于 ELISA, WB, IHC, IF, FCM, IP。

Anti-PKM Antibody (4D256)

还可以

产品编号 TMAH-00983 别名 THBP1, TCB, pyruvate kinase, muscle, PKM2, PK3, OIP3, HEL-S-30, CTHBP

Anti-PKM Antibody (4D256) 是一种 Mouse 抗体，靶向 PKM。Anti-PKM Antibody (4D256) 可用于 ELISA, WB, IHC, IF, FCM, IP。

规格	价格	库存	数量
50 μL	¥ 1,300	5日内发货
100 μL	¥ 2,180	5日内发货

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产品描述	Anti-PKM Antibody (4D256) is a Mouse antibody targeting PKM. Anti-PKM Antibody (4D256) can be used in ELISA, WB, IHC, IF, FCM, IP.
别名	THBP1, TCB, pyruvate kinase, muscle, PKM2, PK3, OIP3, HEL-S-30, CTHBP
Ig Type	IgG1
克隆号	4D256
交叉反应	Human, Rat, Mouse, Rabbit
验证活性	1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate -All lanes: PKM antibody at 1:4000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 58 kDa -Observed band size: 58 KDa -Exposure time: 1min 2. Western Blot -Positive WB detected in: Mouse Heart tissue, Mouse Brain tissue, Mouse Skeletal Muscle tissue -All lanes: PKM antibody at 1:4000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 58 kDa -Observed band size: 58 KDa 3. Western Blot -Positive WB detected in: Rat Heart tissue, Rat Spleen tissue, Rat Brain tissue -All lanes: PKM antibody at 1:4000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 55-60 kDa -Observed band size: 55-60 kDa 4. Western Blot -Positive WB detected in: MCF-7 whole cell lysate at 40µg, 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg, 0.3125µg -All lanes: PKM antibody at 1:4000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 58 kDa -Observed band size: 58 KDa -Exposure time: 5min 5. Western Blot -Positive WB detected in: MCF-7 whole cell lysate -All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 58 kDa -Observed band size: 58 KDa -Exposure time: 5min 6. IHC image of TMAH-00983 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 7. IHC image of TMAH-00983 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 8. IHC image of TMAH-00983 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 9. IHC image of TMAH-00983 diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 10. IHC image of TMAH-00983 diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 11. IHC image of TMAH-00983 diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 12. IHC image of TMAH-00983 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 13. IHC image of TMAH-00983 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 14. IHC image of TMAH-00983 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 15. Immunofluorescence staining of A549 cells with TMAH-00983 at 1:230, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 16. Immunofluorescence staining of Hela cells with TMAH-00983 at 1:230, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 17. Immunofluorescence staining of HepG2 cells with TMAH-00983 at 1:230, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 18. Immunoprecipitating PKM in Hela whole cell lysate -Lane 1: Mouse control IgG instead of TMAH-00983 in Hela whole cell lysate. -Lane 2: TMAH-00983 (1ul) + Hela whole cell lysate (500ug) -Lane 3: Hela whole cell lysate (10ug) For western blotting, the blot was detected with TMAH-00983 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 19. Overlay histogram showing Hela cells stained with TMAH-00983 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. 20. Overlay histogram showing HepG2 cells stained with TMAH-00983 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
应用	ELISA, WB, IHC, IF, FCM, IP
抗体种类	Monoclonal
宿主来源	Mouse
亚细胞定位	Cytoplasm. Nucleus.
构建方式	Hybridoma Monoclonal Antibody
纯化方式	Protein G purified
性状	Liquid
缓冲液	Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
纯度	>95%
研究背景	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. In addition to its role in glycolysis, also regulates transcription. Stimulates POU5F1-mediated transcriptional activation. Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages. Also acts as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs. Plays a general role in caspase independent cell death of tumor cells.