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Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody
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产品编号 TMAB-01494
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Smad1/5 (Ser463, 465)。Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody
还可以产品编号 TMAB-01494
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Smad1/5 (Ser463, 465)。Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Smad1/5 (Ser463, 465). Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat,Pig (predicted:Chicken,Dog,Cow,Horse) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Smad1 5 (Ser463 + Ser465)) Polyclonal Antibody, Unconjugated (TMAB-01494) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Smad1-5 (Ser463+ Ser465)) Polyclonal Antibody, Unconjugated (TMAB-01494) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Cerebrum (Mouse) Lysate at 40 μg Stomach (Mouse) Lysate at 40 μg Primary: Anti-Phospho-Smad1/5 (Ser463 + Ser465) (TMAB-01494) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 62 kDa 4. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Heart (Mouse) Lysate at 40 μg Lane 3: Testis (Mouse) Lysate at 40 μg Lane 4: Skin (Mouse) Lysate at 40 μg Lane 5: Kidney (Mouse) Lysate at 40 μg Lane 6: Cerebrum (Rat) Lysate at 40 μg Lane 7: Testis (Rat) Lysate at 40 μg Lane 8: Skin (Rat) Lysate at 40 μg Lane 9: Kidney (Rat) Lysate at 40 μg Lane 10: Huvec (Human) Cell Lysate at 30 μg Lane 11: A549 (Human) Cell Lysate at 30 μg Lane 12: Hela (Human) Cell Lysate at 30 μg Lane 13: HT1080 (Human) Cell Lysate at 30 μg Lane 14: A431 (Human) Cell Lysate at 30 μg Primary: Anti-Phospho-Smad3 (Ser463 + Ser465) (TMAB-01494) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 54 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. |
| 免疫原 | KLH conjugated Synthesised phosphopeptide: human Smad1/5 around the phosphorylation site of Ser463/465 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | TGF,SMADs,SMADs,Cytoplasmic |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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