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Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody
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产品编号 TMAB-01494
别名 Transforming growth factor-beta-signaling protein 1, Transforming growth factor beta signaling protein 1, TGF beta signaling protein 1, Spinal muscular atrophy distal with upper limb predominance, Smad1/5 (p-Ser463, 465), Smad1/5 (p-S463, 465), SMAD-1, Smad1, SMAD1, SMAD mothers against DPP homolog 1 Drosophila, SMAD family member 1, Sma and Mad related protein 1, p-Smad1/5 (Ser463, 465), p-Smad1/5 (S463, 465), Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, Mad-related protein 1, MADR1, Madr 1, MADH1, MADH 1, Mad related protein 1, MAD mothers against decapentaplegic homolog 1, MAD homolog 1, JV41, JV4 1, JV 41, hSMAD1, hSMAD 1, DwfA, Dwarfin A, BSP1, BSP 1
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Smad1/5 (Ser463, 465)。Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody
还可以产品编号 TMAB-01494 别名 Transforming growth factor-beta-signaling protein 1, Transforming growth factor beta signaling protein 1, TGF beta signaling protein 1, Spinal muscular atrophy distal with upper limb predominance, Smad1/5 (p-Ser463, 465), Smad1/5 (p-S463, 465), SMAD-1, Smad1, SMAD1, SMAD mothers against DPP homolog 1 Drosophila, SMAD family member 1, Sma and Mad related protein 1, p-Smad1/5 (Ser463, 465), p-Smad1/5 (S463, 465), Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, Mad-related protein 1, MADR1, Madr 1, MADH1, MADH 1, Mad related protein 1, MAD mothers against decapentaplegic homolog 1, MAD homolog 1, JV41, JV4 1, JV 41, hSMAD1, hSMAD 1, DwfA, Dwarfin A, BSP1, BSP 1
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Smad1/5 (Ser463, 465)。Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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| 产品描述 | Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Smad1/5 (Ser463, 465). Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| 疾病造模方案 | |
| 别名 | Transforming growth factor-beta-signaling protein 1, Transforming growth factor beta signaling protein 1, TGF beta signaling protein 1, Spinal muscular atrophy distal with upper limb predominance, Smad1/5 (p-Ser463, 465), Smad1/5 (p-S463, 465), SMAD-1, Smad1, SMAD1, SMAD mothers against DPP homolog 1 Drosophila, SMAD family member 1, Sma and Mad related protein 1, p-Smad1/5 (Ser463, 465), p-Smad1/5 (S463, 465), Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, Mad-related protein 1, MADR1, Madr 1, MADH1, MADH 1, Mad related protein 1, MAD mothers against decapentaplegic homolog 1, MAD homolog 1, JV41, JV4 1, JV 41, hSMAD1, hSMAD 1, DwfA, Dwarfin A, BSP1, BSP 1 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat,Pig (predicted:Chicken,Dog,Cow,Horse) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Smad1 5 (Ser463 + Ser465)) Polyclonal Antibody, Unconjugated (TMAB-01494) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Smad1-5 (Ser463+ Ser465)) Polyclonal Antibody, Unconjugated (TMAB-01494) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Cerebrum (Mouse) Lysate at 40 μg Stomach (Mouse) Lysate at 40 μg Primary: Anti-Phospho-Smad1/5 (Ser463 + Ser465) (TMAB-01494) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 62 kDa 4. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Heart (Mouse) Lysate at 40 μg Lane 3: Testis (Mouse) Lysate at 40 μg Lane 4: Skin (Mouse) Lysate at 40 μg Lane 5: Kidney (Mouse) Lysate at 40 μg Lane 6: Cerebrum (Rat) Lysate at 40 μg Lane 7: Testis (Rat) Lysate at 40 μg Lane 8: Skin (Rat) Lysate at 40 μg Lane 9: Kidney (Rat) Lysate at 40 μg Lane 10: Huvec (Human) Cell Lysate at 30 μg Lane 11: A549 (Human) Cell Lysate at 30 μg Lane 12: Hela (Human) Cell Lysate at 30 μg Lane 13: HT1080 (Human) Cell Lysate at 30 μg Lane 14: A431 (Human) Cell Lysate at 30 μg Primary: Anti-Phospho-Smad3 (Ser463 + Ser465) (TMAB-01494) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 54 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. |
| 组织特异性 | Ubiquitous. Highest expression seen in the heart and skeletal muscle. |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1 mg/mL |
| 研究背景 | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. |
| 免疫原 | KLH conjugated Synthesised phosphopeptide: human Smad1/5 around the phosphorylation site of Ser463/465 |
| 抗原种属 | Human |
| 基因名称 | SMAD1 |
| 基因ID | |
| 蛋白名称 | Mothers against decapentaplegic homolog 1 |
| Uniprot ID | |
| 研究领域 | TGF,SMADs,SMADs,Cytoplasmic |
| 功能 | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. |
| 分子量 | Theoretical: 52 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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