Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PKM2 (Tyr105). Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody can be used in ELISA,FCM,IF,IHC-Fr,IHC-P.

IgG

Human,Rat,Rabbit (predicted:Mouse,Dog,Pig,Cow,Horse,GuineaPig)

1. phosphopeptide
non phosphopeptide
2. Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: Rat Thyroid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-NMDAR1 (Ser890) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugati Phospho-PKM2on to the secondary antibody and DAb staining.
4. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Tissue/cell: rat thyroid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
6. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
7. Tissue/cell: human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
8. Blank control: A549. Primary Antibody (green line): Rabbit Anti-Phospho-PKM2 (Tyr105) antibody (TMAB-01482)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test; ELISA: 1:5000-10000

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PKM2 around the phosphorylation site of Tyr105

Human

PKM

Pyruvate kinase PKM

Energy Metabolism,Energy Metabolism,Carbohydrate metabolism,Kinases/Phosphatases,Enzymes,Metabolism of carbohydrates

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.