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Anti-Phospho-NFE2L2 (S40) Antibody (7L44) 是一种 Rabbit 抗体,靶向 Phospho-NFE2L2 (S40)。Anti-Phospho-NFE2L2 (S40) Antibody (7L44) 可用于 WB,IHC,ICC/IF,IP,FCM。
Anti-Phospho-NFE2L2 (S40) Antibody (7L44) 是一种 Rabbit 抗体,靶向 Phospho-NFE2L2 (S40)。Anti-Phospho-NFE2L2 (S40) Antibody (7L44) 可用于 WB,IHC,ICC/IF,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-Phospho-NFE2L2 (Ser40) Antibody (7L44) 是一种 Rabbit 抗体,靶向 Phospho-NFE2L2 (Ser40)。Anti-Phospho-NFE2L2 (Ser40) Antibody (7L44) 可用于 WB,IHC,ICC/IF,IP,FCM。 |
别名 | p-NFE2L2 (Ser40), p-NFE2L2 (S40), NFE2L2 (p-Ser40), NFE2L2 (p-S40) |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of Phospho-Nrf2 (S40) on different lysates using anti-Phospho-Nrf2 (S40) antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: Raji. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin. 5. ICC staining Phospho-Nrf2 (S40) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Phospho-Nrf2 (S40) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Phospho-Nrf2 (S40) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of K562 cells with Phospho-Nrf2 (S40) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; IP: 1:50; FCM: 1:50 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human Nrf2 around the phosphorylation site of S40 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 90 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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