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顶部生物活性储存和溶解信息计算器

Anti-Phospho-mTOR (Ser2481) Antibody (4R8)

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Anti-Phospho-mTOR (Ser2481) Antibody (4R8)
产品编号 TMAH-00930
别名 p-mTOR (Ser2481), p-mTOR (S2481), Phospho-mTOR (S2481), mTOR (p-Ser2481), mTOR (p-S2481), mTOR

Anti-Phospho-mTOR (Ser2481) Antibody (4R8) 是一种抗体，靶向 Phospho-mTOR (Ser2481)。Anti-Phospho-mTOR (Ser2481) Antibody (4R8) 可用于 ELISA, WB, IF。

Anti-Phospho-mTOR (Ser2481) Antibody (4R8)

Anti-Phospho-mTOR (Ser2481) Antibody (4R8)

Rating icon 还可以
Anti-Phospho-mTOR (Ser2481) Antibody (4R8)
产品编号 TMAH-00930 别名 p-mTOR (Ser2481), p-mTOR (S2481), Phospho-mTOR (S2481), mTOR (p-Ser2481), mTOR (p-S2481), mTOR

Anti-Phospho-mTOR (Ser2481) Antibody (4R8) 是一种抗体，靶向 Phospho-mTOR (Ser2481)。Anti-Phospho-mTOR (Ser2481) Antibody (4R8) 可用于 ELISA, WB, IF。

规格价格库存数量
50 μL
¥ 1,310
5日内发货
100 μL
¥ 2,190
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-mTOR (Ser2481) Antibody (4R8) is an antibody targeting Phospho-mTOR (Ser2481). Anti-Phospho-mTOR (Ser2481) Antibody (4R8) can be used in ELISA, WB, IF.
别名p-mTOR (Ser2481), p-mTOR (S2481), Phospho-mTOR (S2481), mTOR (p-Ser2481), mTOR (p-S2481), mTOR
Ig Type
Rabbit IgG
克隆号
4R8
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in A549 whole cell lysate,293 whole cell lysate(treated with Calyculin A or EGF)
-All lanes Phospho-MTOR antibody at 1.33μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 289 KDa
-Observed band size: 289 KDa
2. Immunofluorescence staining of Hela cells with TMAH-00930 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
应用
ELISA
WB
IF
推荐剂量
WB:1:500-1:5000; IF:1:20-1:200.
抗体种类
Monoclonal
亚细胞定位Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome. Cytoplasm. Nucleus, PML body. Microsome membrane. Lysosome membrane. Cytoplasmic vesicle, phagosome.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. This also includes mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3: in the presence of nutrients, mediates phosphorylation of TFEB and TFE3, promoting their cytosolic retention and inactivation. Upon starvation or lysosomal stress, inhibition of mTORC1 induces dephosphorylation and nuclear translocation of TFEB and TFE3, promoting their transcription factor activity. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. Also prevents autophagy by phosphorylating RUBCNL/Pacer under nutrient-rich conditions. Prevents autophagy by mediating phosphorylation of AMBRA1, thereby inhibiting AMBRA1 ability to mediate ubiquitination of ULK1 and interaction between AMBRA1 and PPP2CA. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422'. Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms. Plays an important regulatory role in the circadian clock function; regulates period length and rhythm amplitude of the suprachiasmatic nucleus (SCN) and liver clocks. Phosphorylates SQSTM1, promoting interaction between SQSTM1 and KEAP1 and subsequent inactivation of the BCR(KEAP1) complex.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human Phospho-MTOR (S2481)
抗原种属
Human
基因ID
2475
Uniprot ID
P42345
研究领域
Cell Biology
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

Tyrosine-protein kinase mTORSerine/threonine-protein kinase mTORRAPT1RAPT 1Rapamycin target protein 1Rapamycin and FKBP12 target 1RAFT1RAFT 1pmTOR (Ser2481)pmTOR (S2481)mTOR (pSer2481)mTOR (pS2481)mTOR (phospho-Ser2481)mTOR (phospho-S2481)mTORMechanistic target of rapamycinMammalian target of rapamycin (mTOR)FRAP2FRAP1FRAP 2FRAP 1FRAPFKBP12-rapamycin complex-associated proteinFK506-binding protein 12-rapamycin complex-associated protein 1

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