Anti-Phospho-JAK1 (Tyr1034, 1035) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JAK1 (Tyr1034, 1035). Anti-Phospho-JAK1 (Tyr1034, 1035) Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Pig,Cow)

1. Sample: A549 (Human) cell Lysate at 40 μg
Primary: Anti-p-Jak1 (Tyr1034 + Tyr1035)(TMAB-01439) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDa
2. From 《Cancer Medicine》(2016.6): PublitionDirect effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes , IF:2.5
Author: Noriyoshi Iriyama, Yoshihiro Hatta & Masami Takei
Division of Hematology and Rheumatology, Department of Medicine, Nihon University School of Medicine, Tokyo, Japan
3. Blank control: HUVEC.
Primary Antibody (green line): Rabbit Anti-Phospho-Jak1 antibody (TMAB-01439)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 2μg /Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Jak1 around the phosphorylation site of Tyr1034 + Tyr1035

Human

Signal transducers,Tyrosine kinases,Kinases,STATs,Tyrosine Kinases