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Anti-PHGDH Antibody (7J468)
还可以产品编号 TMAY-02516
Anti-PHGDH Antibody (7J468) 是一种 Mouse 抗体，靶向 PHGDH。Anti-PHGDH Antibody (7J468) 可用于 FCM,ICC/IF。
Anti-PHGDH Antibody (7J468)
Anti-PHGDH Antibody (7J468)
还可以产品编号 TMAY-02516
Anti-PHGDH Antibody (7J468) 是一种 Mouse 抗体，靶向 PHGDH。Anti-PHGDH Antibody (7J468) 可用于 FCM,ICC/IF。
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产品介绍
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抗原信息
| 产品描述 | Anti-PHGDH Antibody (7J468) is a Mouse antibody targeting PHGDH. Anti-PHGDH Antibody (7J468) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 7J468 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human PHGDH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human PHGDH monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human PHGDH expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PHGDH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PGDH / PHGDH (rh PGDH / PHGDH; TMPY-02801; O43175; Met1-Phe533). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human PGDH / PHGDH protein (TMPY-02801) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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