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Anti-PGK1 Antibody (1H456)
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产品编号 TMAC-03177
别名 Primer recognition protein 2, Phosphoglycerate kinase 1, PGKA, PGK1, Cell migration-inducing gene 10 protein
Anti-PGK1 Antibody (1H456) 是一种 Rabbit 抗体，靶向 PGK1。Anti-PGK1 Antibody (1H456) 可用于 WB,ICC/IF,IP,FCM。
Anti-PGK1 Antibody (1H456)
Anti-PGK1 Antibody (1H456)
还可以产品编号 TMAC-03177 别名 Primer recognition protein 2, Phosphoglycerate kinase 1, PGKA, PGK1, Cell migration-inducing gene 10 protein
Anti-PGK1 Antibody (1H456) 是一种 Rabbit 抗体，靶向 PGK1。Anti-PGK1 Antibody (1H456) 可用于 WB,ICC/IF,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
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偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PGK1 Antibody (1H456) is a Rabbit antibody targeting PGK1. Anti-PGK1 Antibody (1H456) can be used in WB,ICC/IF,IP,FCM. |
| 别名 | Primer recognition protein 2, Phosphoglycerate kinase 1, PGKA, PGK1, Cell migration-inducing gene 10 protein |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PGK1 on different lysates using anti-PGK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: HepG2, Lane 3: MCF-7. 2. ICC staining PGK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. Flow cytometric analysis of Hela cells with PGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Phosphoglycerate kinases 1/2 (PGK1/2, ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) are somatically expressed, glycolytic enzymes that catalyze the transfer of a phosphoryl group from the acyl phosphate of 1,3-bisphosphoglycerate to ADP, thereby forming ATP and 3-phosphoglycerate. The human PGK gene is interrupted by 10 introns and spans 23 kilobases, and is X chromosome-linked at position Xq21.1, a region implicated in prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. In addition to influencing glycolysis, the PGK1 is secreted by tumor cells and contributes to proliferative angiogenic processes as a disulfide reductase. PGK1 mediated reduction of disulphide bonds in the serine proteinase plasmin initiates the release of the tumor blood vessel inhibitor angiostatin, an event that is critical for blood vessel formation or angiogenesis in tumor expansion and metastasis. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 45 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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