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Anti-Peroxiredoxin 6 Antibody (5E148)

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产品编号 TMAY-01802

Anti-Peroxiredoxin 6 Antibody (5E148) 是一种 Mouse 抗体,靶向 Peroxiredoxin 6。Anti-Peroxiredoxin 6 Antibody (5E148) 可用于 ELISA,IHC-P,IP。

Anti-Peroxiredoxin 6 Antibody (5E148)

Anti-Peroxiredoxin 6 Antibody (5E148)

Rating icon 还可以
产品编号 TMAY-01802

Anti-Peroxiredoxin 6 Antibody (5E148) 是一种 Mouse 抗体,靶向 Peroxiredoxin 6。Anti-Peroxiredoxin 6 Antibody (5E148) 可用于 ELISA,IHC-P,IP。

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产品介绍

生物活性
产品描述
Anti-Peroxiredoxin 6 Antibody (5E148) is a Mouse antibody targeting Peroxiredoxin 6. Anti-Peroxiredoxin 6 Antibody (5E148) can be used in ELISA,IHC-P,IP.
Ig Type
Monoclonal Mouse IgG1
克隆号
5E148
交叉反应
Human
验证活性
1. Immunochemical staining of human PRDX6 in human brain with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to neuroglia cells.

2. PRDX6 was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-2 µL anti-PRDX6 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-PRDX6 mouse monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 26 kDa.

-Observed band size: 26 kDa
应用
ELISA,IHC-P,IP
推荐剂量
ELISA: 1:1000-1:2000; IHC-P: 1:50-1:200; IP: 4-6 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Peroxiredoxin 6 / PRDX6 (rh Peroxiredoxin 6 / PRDX6; TMPY-02201; P30041; Met 1-Pro 224). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0
研究背景PRDX6, a member of antioxidant protein superfamily, plays an important role in oxidative stress, catabolism of lipids and phospholipid lipisomes. Peroxiredoxin 6 (PRDX6) is involved in redox regulation of the cell and is thought to be protective against oxidant injury. Peroxiredoxin 6 (PRDX6) is a bifunctional protein with both glutathione peroxidase (GPx) and iPLA2 activities,which are concomitantly increased with the expression of PRDX6. PRDX6 promoted lung tumor growth in an in vivo allograft model.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
FITC/PE
可提供抗体种类
3
抗原信息
免疫原
Recombinant Human Peroxiredoxin 6 / PRDX6 protein (TMPY-02201)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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