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Anti-PDGF R alpha/PDGFRA Antibody (8J71)
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产品编号 TMAC-03117
别名 RHEPDGFRA, PDGFRA, PDGFR-2, PDGFR2, PDGF R α/PDGFRA Protein, Human, Recombinant (His & Avi), Biotinylated, MGC74795, CD140A
Anti-PDGF R alpha/PDGFRA Antibody (8J71) 是一种 Rabbit 抗体，靶向 PDGF R alpha/PDGFRA。Anti-PDGF R alpha/PDGFRA Antibody (8J71) 可用于 WB,ICC,IHC,FCM。
Anti-PDGF R alpha/PDGFRA Antibody (8J71)
Anti-PDGF R alpha/PDGFRA Antibody (8J71)
还可以产品编号 TMAC-03117 别名 RHEPDGFRA, PDGFRA, PDGFR-2, PDGFR2, PDGF R α/PDGFRA Protein, Human, Recombinant (His & Avi), Biotinylated, MGC74795, CD140A
Anti-PDGF R alpha/PDGFRA Antibody (8J71) 是一种 Rabbit 抗体，靶向 PDGF R alpha/PDGFRA。Anti-PDGF R alpha/PDGFRA Antibody (8J71) 可用于 WB,ICC,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
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偶联与修饰
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| 产品描述 | Anti-PDGF R alpha/PDGFRA Antibody (8J71) is a Rabbit antibody targeting PDGF R alpha/PDGFRA. Anti-PDGF R alpha/PDGFRA Antibody (8J71) can be used in WB,ICC,IHC,FCM. |
| 别名 | RHEPDGFRA, PDGFRA, PDGFR-2, PDGFR2, PDGF R α/PDGFRA Protein, Human, Recombinant (His & Avi), Biotinylated, MGC74795, CD140A |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PDGFR alpha on NIH/3T3 cells lysates using anti-PDGFR alpha antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin. 5. ICC staining PDGFR alpha in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining PDGFR alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with PDGFR alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and -β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two α receptors, PDGF-AB induces dimerization of αα and αβ and PDGF-BB induces the formation of three types of dimers, αα, αβ and ββ. Translocation of the PDGFR-β gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 123 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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