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Anti-PARK7/DJ-1 Antibody (9L104)
还可以产品编号 TMAY-02369
别名 parkinson protein 7, HEL-S-67p, DJ-1, DJ1
Anti-PARK7/DJ-1 Antibody (9L104) 是一种 Rabbit 抗体，靶向 PARK7/DJ-1。Anti-PARK7/DJ-1 Antibody (9L104) 可用于 WB,FCM,ICC/IF,IP。
Anti-PARK7/DJ-1 Antibody (9L104)
Anti-PARK7/DJ-1 Antibody (9L104)
还可以产品编号 TMAY-02369 别名 parkinson protein 7, HEL-S-67p, DJ-1, DJ1
Anti-PARK7/DJ-1 Antibody (9L104) 是一种 Rabbit 抗体，靶向 PARK7/DJ-1。Anti-PARK7/DJ-1 Antibody (9L104) 可用于 WB,FCM,ICC/IF,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μL | ¥ 790 | 5日内发货 | |
| 100 μL | ¥ 2,289 | 5日内发货 |
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| 产品描述 | Anti-PARK7/DJ-1 Antibody (9L104) is a Rabbit antibody targeting PARK7/DJ-1. Anti-PARK7/DJ-1 Antibody (9L104) can be used in WB,FCM,ICC/IF,IP. |
| 别名 | parkinson protein 7, HEL-S-67p, DJ-1, DJ1 |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 9L104 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human PARK7 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PARK7 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human PARK7 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PARK7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. Anti-PARK7 rabbit monoclonal antibody at 1:1000 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:20 kDa. -Observed band size:24 kDa. 4. PARK7 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -Lane C:0.5 mg 293T Whole Cell Lysate. -2 µL anti-PARK7 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PARK7 rabbit monoclonal antibody, at 1:1000 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 20 kDa. -Observed band size: 20 kDa |
| 应用 | WB,FCM,ICC/IF,IP |
| 推荐剂量 | WB: 1:1000-1:5000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human PARK7 / DJ-1 (rh PARK7 / DJ-1; TMPY-02043; Q99497-1; Met1-Asp189). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Parkinson's disease locus DJ-1 (PARK7) is a differentially expressed transcript. DJ-1 plays a physiologic role in protection of erythroid cells from oxidant damage, a function unmasked in the context of oxidative stress. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease (PD). DJ-1/p53 interactions contribute to apoptosis resistance in clonal myeloid cells and may serve as a prognostic marker in patients with myelodysplastic syndromes (MDS). DJ-1 regulates redox signaling kinase pathways and acts as a transcriptional regulator of antioxidative gene batteries. Therefore, DJ-1 is an important redox-reactive signaling intermediate controlling oxidative stress after ischemia, upon neuroinflammation, and during age-related neurodegenerative processes. Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human PARK7 / DJ-1 protein (TMPY-02043) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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