Anti-PAFR/PAF Receptor Polyclonal Antibody is a Rabbit antibody targeting PAFR/PAF Receptor. Anti-PAFR/PAF Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Rabbit)

1. Sample: LympH(Mouse) Lysate at 30 μg
Primary: Anti-RAFR/PAF (TMAB-01326) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
2. Blank control: 293T cells (blue). Primary Antibody: Rabbit Anti-PAFR/PAF antibody (TMAB-01326), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-01326, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
3. Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PTAFR Polyclonal Antibody, Unconjugated (TMAB-01326) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PTAFR Polyclonal Antibody, Unconjugated (TMAB-01326) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Sample:
Lane1: Lung (Rat) Lysate at 30 μg
Lane2: Brain (Rat) Lysate at 30 μg
Primary: Anti-PTAFR (TMAB-01326) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution;
Predicted band size: 38 kDa
Observed band size: 38 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1µg/Test

Polyclonal

KLH conjugated synthetic peptide: human PAFR

Human

Blood,Platelets,Macrophage / Inflamm.,GPCR