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Anti-N Cadherin Antibody (3E575)
还可以产品编号 TMAY-01507
Anti-N Cadherin Antibody (3E575) 是一种 Rabbit 抗体，靶向 N Cadherin。Anti-N Cadherin Antibody (3E575) 可用于 WB,ELISA,FCM,ICC/IF。
Anti-N Cadherin Antibody (3E575)
Anti-N Cadherin Antibody (3E575)
还可以产品编号 TMAY-01507
Anti-N Cadherin Antibody (3E575) 是一种 Rabbit 抗体，靶向 N Cadherin。Anti-N Cadherin Antibody (3E575) 可用于 WB,ELISA,FCM,ICC/IF。
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生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-N Cadherin Antibody (3E575) is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody (3E575) can be used in WB,ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 3E575 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human CDH2 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CDH2 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to membrane. 2. Flow cytometry analysis of N-Cadherin on HeLa cells. HeLa cells were harvested with (Left) or without (Right) trypsinization [please note, the epitope is sensitive to trypsin] and stained with either purified Rabbit IgG isotype control (dashed line) or Rabbit Anti-Human CD325 antibody [solid line, and Clone 1T666 (upper), Clone 3E575 (bottom)] at matching concentrations , then a FITC-conjugated second step antibody. 3. Anti-CDH2 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:99 kDa. -Observed band size:130 kDa. 4. Anti-N Cadherin rabbit monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell Lysate. -Lane B: N Cadherin konckout 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:99 kDa. -Observed band size:130 kDa(Validation Experiment) |
| 应用 | WB,ELISA,FCM,ICC/IF |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human N-Cadherin / CD325 / CDH2 (rh N-Cadherin / CD325 / CDH2; TMPY-01143; NP_001783.2; Met 1-Ala 724). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Cadherins are calcium-dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass transmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/FITC/PE |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Human N-Cadherin / CD325 / CDH2 Protein (TMPY-01143) |
| 抗原种属 | Human |
| 研究领域 | Early Mesodermal Lineage Markers |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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