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Anti-MTCO2 Antibody (5B251)
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产品编号 TMAC-02671
别名 MT-CO2, MTCO2, MTCO 2, MT CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, Cytochrome c oxidase II, COXII, COX2_HUMAN, COX2, COII
Anti-MTCO2 Antibody (5B251) 是一种 Rabbit 抗体，靶向 MTCO2。Anti-MTCO2 Antibody (5B251) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-MTCO2 Antibody (5B251)
Anti-MTCO2 Antibody (5B251)
还可以产品编号 TMAC-02671 别名 MT-CO2, MTCO2, MTCO 2, MT CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, Cytochrome c oxidase II, COXII, COX2_HUMAN, COX2, COII
Anti-MTCO2 Antibody (5B251) 是一种 Rabbit 抗体，靶向 MTCO2。Anti-MTCO2 Antibody (5B251) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-MTCO2 Antibody (5B251) is a Rabbit antibody targeting MTCO2. Anti-MTCO2 Antibody (5B251) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | MT-CO2, MTCO2, MTCO 2, MT CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, Cytochrome c oxidase II, COXII, COX2_HUMAN, COX2, COII |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of MTCO2 on different lysates using anti-MTCO2 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7, Lane 2: THP-1, Lane 3: Hela. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin. 3. ICC staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cytochrome c oxidase subunit II (COX2), also designated COII, MTCO2 or oxidative phosphorylation (OxPhos) complex IV, subunit II, is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electrochemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded. Defects in COX2 are associated with tumor formation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 25 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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