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Anti-MMP-13 Antibody (2L744) 是一种 Rabbit 抗体,靶向 MMP-13。Anti-MMP-13 Antibody (2L744) 可用于 WB,ICC/IF,IHC,FCM。
Anti-MMP-13 Antibody (2L744) 是一种 Rabbit 抗体,靶向 MMP-13。Anti-MMP-13 Antibody (2L744) 可用于 WB,ICC/IF,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-MMP-13 Antibody (2L744) is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Antibody (2L744) can be used in WB,ICC/IF,IHC,FCM. |
别名 | MMP13, Matrix metalloproteinase-13 (MMP-13), Collagenase 3 |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of MMP13 on MCF-7 cells lysates using anti-MMP13 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MMP13 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MMP13 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP13 antibody. Counter stained with hematoxylin. 5. ICC staining MMP13 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining MMP13 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining MMP13 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with MMP13 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-13 (also designated collagenase-3) is produced by breast carcinomas and degrades collagen types I, II and III. MMP-13 has wide substrate specificity, and its physiologic expression is limited to situations in which rapid and effective remodeling of collagenous ECM takes place, such as fetal bone development and adult bone remodeling. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 54 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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