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顶部生物活性储存和溶解信息计算器

Anti-MMP-12 Antibody (2O982)

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产品编号 TMAH-00764

别名 HME

Anti-MMP-12 Antibody (2O982) 是一种抗体，靶向 MMP-12。Anti-MMP-12 Antibody (2O982) 可用于 ELISA,IHC,WB。

Anti-MMP-12 Antibody (2O982)

Anti-MMP-12 Antibody (2O982)

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Rating icon 还可以
产品编号 TMAH-00764 别名 HME

Anti-MMP-12 Antibody (2O982) 是一种抗体，靶向 MMP-12。Anti-MMP-12 Antibody (2O982) 可用于 ELISA,IHC,WB。

规格价格库存数量
50 μL
¥ 1,315
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100 μL
¥ 2,195
5日内发货
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产品介绍

生物活性
产品描述
Anti-MMP-12 Antibody (2O982) is an antibody targeting MMP-12. Anti-MMP-12 Antibody (2O982) can be used in ELISA,IHC,WB.
别名HME
Ig Type
Rabbit IgG
克隆号
2O982
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate
-All lanes: MMP12 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 55 kDa
-Observed band size: 54, 45 kDa
2. IHC image of TMAH-00764 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用ELISAIHCWB
推荐剂量
WB:1:500-1:5000; IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Secreted, extracellular space, extracellular matrix.
组织特异性Found in alveolar macrophages but not in peripheral blood monocytes.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human MMP12
抗原种属
Human
基因名称
MMP12
基因ID
4321
Uniprot ID
P39900
研究领域
Cancer, Cardiovascular, Signal transduction
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

MMP-12MMP12MMP 12MMEMEMatrix metalloproteinase-12 (MMP-12)Macrophage metalloelastaseMacrophage elastase (ME;hME)hME

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4个月前
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