Anti-MAX Antibody (6Q204)

Name: Anti-MAX Antibody (6Q204)
Brand: TargetMol
SKU: TMAY-02427
Rating: 4.5 (1 reviews)

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产品编号 TMAY-02427

Anti-MAX Antibody (6Q204) 是一种 Rabbit 抗体，靶向 MAX。Anti-MAX Antibody (6Q204) 可用于 WB,ELISA,FCM,ICC/IF,IP。

Anti-MAX Antibody (6Q204)

还可以

产品编号 TMAY-02427

Anti-MAX Antibody (6Q204) 是一种 Rabbit 抗体，靶向 MAX。Anti-MAX Antibody (6Q204) 可用于 WB,ELISA,FCM,ICC/IF,IP。

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生物活性

产品描述	Anti-MAX Antibody (6Q204) is a Rabbit antibody targeting MAX. Anti-MAX Antibody (6Q204) can be used in WB,ELISA,FCM,ICC/IF,IP.
Ig Type	Monoclonal Rabbit IgG
克隆号	6Q204
交叉反应	Human
验证活性	1. Immunofluorescence staining of Human MAX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MAX monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus. 2. Flow cytometric analysis of Human MAX expression on Raji cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. MAX was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate -0.5 µL anti-MAX rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-MAX rabbit monoclonal antibody, at 1:1000 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 23 kDa. -Observed band size: 23 kDa. 4. Anti-MAX rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:18 kDa. -Observed band size:20 kDa(We are unsure as to the identity of these extra bands.)
应用	WB,ELISA,FCM,ICC/IF,IP
推荐剂量	WB: 1:500-1:1000; ELISA: 1:25000-1:50000; ICC-IF: 1:100-1:500; FCM: 1:100-1:500; IP: 0.2-1 μL/mg of lysate
抗体种类	Monoclonal
宿主来源	Rabbit
构建方式	This antibody was obtained from a rabbit immunized with purified, recombinant Human MAX / MYC associated factor X (rh MAX / MYC associated factor X ; TMPY-02888; NP_002373; Met1-Ser160).
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0
研究背景	MYC associated factor X contains 1 basic helix-loop-helix (bHLH) domain and belongs to the MAX family. It is highly expressed in the brain, heart, and lung while lower levels are seen in the liver, kidney, and skeletal muscle. MYC associated factor X can form homodimers and heterodimers with other family members, which include Mad, Mxi1, and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation, and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. MYC associated factor X may also repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Multiple alternatively spliced transcript variants have been described for MYC associated factor X gene but the full-length nature for some of them is unknown.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Human MAX / MYC associated factor X protein (TMPY-02888)
抗原种属	Human
研究领域	Apoptosis Transcription Factors and Regulators

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.