Anti-LTF/Lactoferrin Polyclonal Antibody is a Rabbit antibody targeting LTF/Lactoferrin. Anti-LTF/Lactoferrin Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Sheep)

1. Sample: Breast milk (Human) Lysate at 40 μg
Primary: Anti-Lactoferrin (TMAB-01086) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa
2. Blank control: U-937. Primary Antibody (green line): Rabbit Anti-Lactoferrin antibody (TMAB-01086)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. U-937 cells were fixed with 4% PFA for 10 min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Antibody (TMAB-01086) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

WB: 1:500-2000; FCM: 1ug/test

Polyclonal

KLH conjugated synthetic peptide: human Lactoferrin

Human

Apoptosis,Apoptosis,Serum Proteins