Anti-LAPTM5 Polyclonal Antibody is a Rabbit antibody targeting LAPTM5. Anti-LAPTM5 Polyclonal Antibody can be used in FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Horse,Rabbit)

Blank control (black line): U937. Primary Antibody (green line): Rabbit Anti-LAPTM5 antibody (TMAB-01048)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human LAPTM5

Human