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顶部生物活性储存和溶解信息计算器

Anti-KRT7 Antibody (4X26)

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产品编号 TMAC-01093

别名 Type-II keratin Kb7, type II cytoskeletal 7, SCL, Sarcolectin, KRT7, Keratin-7 (K7), Keratin, type II cytoskeletal 7, Cytokeratin-7 (CK-7)

Anti-KRT7 Antibody (4X26) 是一种 Rabbit 抗体，靶向 KRT7。Anti-KRT7 Antibody (4X26) 可用于 FCM,ICC/IF,IHC,IP,WB。

Anti-KRT7 Antibody (4X26)

Anti-KRT7 Antibody (4X26)

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产品编号 TMAC-01093 别名 Type-II keratin Kb7, type II cytoskeletal 7, SCL, Sarcolectin, KRT7, Keratin-7 (K7), Keratin, type II cytoskeletal 7, Cytokeratin-7 (CK-7)

Anti-KRT7 Antibody (4X26) 是一种 Rabbit 抗体，靶向 KRT7。Anti-KRT7 Antibody (4X26) 可用于 FCM,ICC/IF,IHC,IP,WB。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-KRT7 Antibody (4X26) is a Rabbit antibody targeting KRT7. Anti-KRT7 Antibody (4X26) can be used in FCM,ICC/IF,IHC,IP,WB.
别名Type-II keratin Kb7, type II cytoskeletal 7, SCL, Sarcolectin, KRT7, Keratin-7 (K7), Keratin, type II cytoskeletal 7, Cytokeratin-7 (CK-7)
Ig Type
IgG
克隆号
4X26
交叉反应
Human
验证活性
1. Western blot analysis of Cytokeratin 7 on different lysates using anti-Cytokeratin 7 antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: HepG2, Lane 3: SK-BR-3, Lane 4: SW1990.
2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.
4. ICC staining Cytokeratin 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Cytokeratin 7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Cytokeratin 7 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Hela cells with Cytokeratin 7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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应用FCMICC/IFIHCIPWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P08729
化学信息
分子量Theoretical: 51 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

Type-II keratin Kb 7KRT 7Keratin7 (K7)Cytokeratin7 (CK7)

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