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Anti-KRT20 Antibody (7P648)
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产品编号 TMAC-01085
别名 type I cytoskeletal 20, Protein IT, KRT 20, Keratin-20, Keratin, K20, Cytokeratin-20, CK-20
Anti-KRT20 Antibody (7P648) 是一种 Rabbit 抗体，靶向 KRT20。Anti-KRT20 Antibody (7P648) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-KRT20 Antibody (7P648)
Anti-KRT20 Antibody (7P648)
还可以产品编号 TMAC-01085 别名 type I cytoskeletal 20, Protein IT, KRT 20, Keratin-20, Keratin, K20, Cytokeratin-20, CK-20
Anti-KRT20 Antibody (7P648) 是一种 Rabbit 抗体，靶向 KRT20。Anti-KRT20 Antibody (7P648) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
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抗原信息
化学信息
| 产品描述 | Anti-KRT20 Antibody (7P648) is a Rabbit antibody targeting KRT20. Anti-KRT20 Antibody (7P648) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | type I cytoskeletal 20, Protein IT, KRT 20, Keratin-20, Keratin, K20, Cytokeratin-20, CK-20 |
| Ig Type | IgG |
| 交叉反应 | Human,Rat |
| 验证活性 | 1. Western blot analysis of Cytokeratin 20 on different cell lysates using anti-Cytokeratin 20 antibody at 1/1,000 dilution. Positive control: Lane 1: Lovo, Lane 2: HCT116, Lane 3: CRC. 2. Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin. 4. ICC staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of CRC cells with Cytokeratin 20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:10-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epi-thelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition, they aid in the characterization of malignant tumors. Cytokeratin 20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract, and Cytokeratin 20 is a useful marker of pancreatic and colorectal cancer. Cytokeratin 20 is also helpful in distinguishing different types of highly related carcinomas, such as renal oncocytomas from renal cell carcinomas. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 44 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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