Anti-JAK1 Polyclonal Antibody is a Rabbit antibody targeting JAK1. Anti-JAK1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human

1. Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-JAK1 antibody (TMAB-01002)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
LOVO (Human) Cell Lysate at 30 μg
Siha (Human) Cell Lysate at 30 μg
Primary: Anti-JAK1 (TMAB-01002) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 120 kDa

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human JAK1

Human

Signal transducers,Tyrosine kinases,Kinases,STATs,Tyrosine Kinases