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Anti-IDH2 Antibody (3T725) 是一种 Rabbit 抗体,靶向 IDH2。Anti-IDH2 Antibody (3T725) 可用于 WB,IHC,FCM。
Anti-IDH2 Antibody (3T725) 是一种 Rabbit 抗体,靶向 IDH2。Anti-IDH2 Antibody (3T725) 可用于 WB,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-IDH2 Antibody (3T725) is a Rabbit antibody targeting IDH2. Anti-IDH2 Antibody (3T725) can be used in WB,IHC,FCM. |
别名 | Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, mNADP-IDH, Isocitrate dehydrogenase 2 (NADP+), mitochondrial, Isocitrate dehydrogenase [NADP], mitochondrial, IDPM, IDP, IDHP_HUMAN, IDHM, IDH 2, IDH, ICD-M, D2HGA2 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of IDH2 on different cell lysate using anti-IDH2 antibody at 1/1,000 dilution. Positive control: Line 1: Mouse heart, Line 2: Rat heart, Line 3: 293T. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IDH2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IDH2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-IDH2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-IDH2 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-IDH2 antibody. Counter stained with hematoxylin. 7. Flow cytometric analysis of MCF-7 cells with IDH2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | IDH2 (isocitrate dehydrogenase 2 (NADP+), mitochondrial), also designated NADP+-specific ICDH; isocitrate dehydrogenase, mitochondrial; and oxalosuccinate decarboxylase, is a 452 amino acid enzyme encoded by the human gene IDH2. IDH2 belongs to the isocitrate and isopropylmalate dehydrogenases family and contains two nucleotide binding regions. IDH2 is involved in the reduction of NADP+ to NADPH and maintains the supply of glutathione (GSH) in mitochondria. It is believed to play a role in intermediary metabolism and energy production. IDH2 also tightly associates with the pyruvate dehydrogenase complex. IDH2 is found in the mitochondrion as a homodimer and can bind one magnesium or manganese ion per subunit. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 45 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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