Anti-human CD73 Antibody (1O588) is a Mouse antibody targeting human CD73. Anti-human CD73 Antibody (1O588) can be used in FCM.

Mouse IgG1, k

1O588

Human

Blank control: A375.
Primary Antibody (green line): Mouse Anti-human CD73 antibody (TMAB-07419)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Monoclonal

Nt5e

5'-nucleotidase

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.