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Anti-HNRNPC Antibody (2T80) 是一种 Rabbit 抗体,靶向 HNRNPC。Anti-HNRNPC Antibody (2T80) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-HNRNPC Antibody (2T80) 是一种 Rabbit 抗体,靶向 HNRNPC。Anti-HNRNPC Antibody (2T80) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-HNRNPC Antibody (2T80) is a Rabbit antibody targeting HNRNPC. Anti-HNRNPC Antibody (2T80) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | SNRPC, Nuclear ribonucleoprotein particle C2 protein, Nuclear ribonucleoprotein particle C1 protein, MGC131677, MGC117353, MGC105117, MGC104306, Hnrnpc, hnRNP C1/C2, hnRNP C1 / hnRNP C2, HNRNP, Heterogeneous nuclear ribonucleoproteins C1/C2, Heterogeneous nuclear ribonucleoprotein C, C2, C1/C2, C1 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat,zebrafish |
验证活性 | 1. Western blot analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: MCF-7, Lane 3: HepG2. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. 7. ICC staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Hela cells with hnRNP C1+C2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and, in particular, the hnRNP A1 protein is one of the major pre-mRNA/mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however, some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, in-cluding hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the pre-mRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
分子量 | Theoretical: 42 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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