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Anti-HNRNPA1 Antibody (4Q19) 是一种 Rabbit 抗体,靶向 HNRNPA1。Anti-HNRNPA1 Antibody (4Q19) 可用于 WB,ICC,IHC,FCM。
Anti-HNRNPA1 Antibody (4Q19) 是一种 Rabbit 抗体,靶向 HNRNPA1。Anti-HNRNPA1 Antibody (4Q19) 可用于 WB,ICC,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-HNRNPA1 Antibody (4Q19) is a Rabbit antibody targeting HNRNPA1. Anti-HNRNPA1 Antibody (4Q19) can be used in WB,ICC,IHC,FCM. |
别名 | Single-strand RNA-binding protein, HNRPA1, HNRNPA1, hnRNP core protein A1, hnRNP A1, Heterogeneous nuclear ribonucleoprotein A1, Helix-destabilizing protein |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of hnRNP A1 on HepG2 (1) and PC-12 (2) cell using anti-hnRNP A1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-hnRNP A1 antibody. Counter stained with hematoxylin. 3. ICC staining hnRNP A1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining hnRNP A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining hnRNP A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Jurkat cells with hnRNP A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
应用 | |
推荐剂量 | WB: 1:500-2000; ICC: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. The A/B subfamily of hnRNPs include A1, A2/B1, A3 and A0, and in Xenopus, hnRNP A1, A2 and A3 are ubiquitously expressed throughout development as well as in adult tissues. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The hnRNP A0 gene is distinct from the other A/B family members, and it encodes a low-abundance protein, which is implicated in mRNA stability. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 33 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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