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顶部产品介绍偶联与修饰储存和溶解信息计算器

Anti-His tag (C-term) Antibody (2B404)

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货号 TMAS-00128

别名 HisTag (C-term), His Tag (Cterm)

Anti-His tag (C-term) Antibody (2B404) 是一种 Rabbit 抗体，靶向 His tag (C-term)。Anti-His tag (C-term) Antibody (2B404) 可用于 ELISA, FCM, ICC/IF, IP, WB。

Anti-His tag (C-term) Antibody (2B404)

Anti-His tag (C-term) Antibody (2B404)

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Rating icon 还可以
货号 TMAS-00128 别名 HisTag (C-term), His Tag (Cterm)

Anti-His tag (C-term) Antibody (2B404) 是一种 Rabbit 抗体，靶向 His tag (C-term)。Anti-His tag (C-term) Antibody (2B404) 可用于 ELISA, FCM, ICC/IF, IP, WB。

规格价格库存数量
40 μg
¥ 3,218
5日内发货
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产品介绍

生物活性
产品描述
Anti-His tag (C-term) Antibody (2B404) is a Rabbit antibody targeting His tag (C-term). Anti-His tag (C-term) Antibody (2B404) can be used in ELISA, FCM, ICC/IF, IP, WB.
别名HisTag (C-term), His Tag (Cterm)
Ig Type
IgG
克隆号
2B404
特异性
His tags placed at C-terminal and internal regions of His-fusion proteins. Not recommended for N-terminal His-fusion protein detection.
验证活性
1. Sensitivity and specificity of Anti-His tag (C-term) Antibody (2B404) (TMAS-00128) to different quantities of a recombinant His6-tagged protein was determined by Western blot. The primary antibody was Anti-His tag (C-term) Antibody (2B404) (TMAS-00128) at 0.2 μg/mL concentration. The secondary antibody was Goat anti-rabbit IgG (H&L) [IRDye800] at 0.125 μg/mL concentration.
2. Cross-reactivity of Anti-His tag (C-term) Antibody (2B404) (TMAS-00128) was determined by Western blot. The primary antibodies were (A) Anti-His tag (C-term) Antibody (2B404) (TMAS-00128) at 1μg/mL concentration; and (B) Rabbit IgG (Negative control) at 1μg/mL concentration. The secondary antibody was Goat anti-Rabbit IgG (H&L) [IRDye800] at 0.125μg/mL concentration.
3. Cell lysates containing over-expressed His-tagged protein were used for immunoprecipitation using Anti-His tag (C-term) Antibody (2B404) (TMAS-00128) and the result was analyzed by Western blot.
Lane 1: Immunoprecipitation with Anti-His tag (C-term) Antibody (2B404) (TMAS-00128) (4 μg)
Lane 2: Negative control – IP with Rabbit IgG (4 μg)
Lane 3: Positive control containing His-tagged protein
The primary antibody for Western blot detection was Anti-His tag (C-term) Antibody (2B404) (TMAS-00128) at 0.2 μg/mL concentration.
The secondary antibody was Goat anti-rabbit IgG (H&L) [IRDye800] at 0.125 μg/mL concentration.
应用ELISAFCMICC/IFIPWB
推荐剂量
ELISA: 0.05-0.2 μg/ml; FCM: 1 μg/ml; ICC/IF: 1 μg/ml; IP: 2 μg/ml; WB: 0.1-0.2 μg/ml
抗体种类
Monoclonal
宿主来源Rabbit
纯化方式Protein A affinity column
性状Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
缓冲液Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
研究背景0
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
KLH conjugated synthetic peptide: HHHHHH
储存&溶解度
储存方式The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储-20°C for 1 year

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