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别名 GSTtag
Anti-GST tag Polyclonal Antibody 3 是一种 Rabbit 抗体,靶向 GST tag。Anti-GST tag Polyclonal Antibody 3 可用于 ELISA, WB。
Anti-GST tag Polyclonal Antibody 3 是一种 Rabbit 抗体,靶向 GST tag。Anti-GST tag Polyclonal Antibody 3 可用于 ELISA, WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 40 μg | ¥ 1,609 | 5日内发货 |
| 产品描述 | Anti-GST tag Polyclonal Antibody 3 is a Rabbit antibody targeting GST tag. Anti-GST tag Polyclonal Antibody 3 can be used in ELISA, WB. |
| 别名 | GSTtag |
| Ig Type | IgG |
| 反应种属 | Schistosoma japonicum |
| 特异性 | GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems |
| 验证活性 | 1. Western blot analysis of immunoprecipitates from GST fusion protein lysates. GST-tagged protein was immunoprecipitated with Rabbit Anti-GST tag Polyclonal Antibody 3 (TMAS-00140) and specificity was confirmed by immunprecipitation with rabbit IgG. Western blot was performed using Rabbit Anti-GST tag Polyclonal Antibody 3 (TMAS-00140). 2. Western blot analysis of GST fusion protein using 1 μg/ml Rabbit Anti-GST tag Polyclonal Antibody 3 (TMAS-00140). |
| 应用 | ELISAWB |
| 推荐剂量 | ELISA: 0.05-0.5 µg/ml; WB: 0.1-1.0 µg/ml |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 纯化方式 | Affinity-chromatography |
| 性状 | Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| 缓冲液 | Supplied in PBS, pH 7.4, containing 0.02% sodium azide |
| 研究背景 | Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Glutathione-S-Transferase from Schistosoma japonicum |
| 储存方式 | The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |