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Anti-GPX1 Antibody (3M540)
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产品编号 TMAC-01687
Anti-GPX1 Antibody (3M540) 是一种 Mouse 抗体，靶向 GPX1。Anti-GPX1 Antibody (3M540) 可用于 WB,ICC,IHC,FCM。
Anti-GPX1 Antibody (3M540)
Anti-GPX1 Antibody (3M540)
还可以产品编号 TMAC-01687
Anti-GPX1 Antibody (3M540) 是一种 Mouse 抗体，靶向 GPX1。Anti-GPX1 Antibody (3M540) 可用于 WB,ICC,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,980 | 5日内发货 | |
| 100 μL | ¥ 2,985 | 5日内发货 |
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| 产品描述 | Anti-GPX1 Antibody (3M540) is a Mouse antibody targeting GPX1. Anti-GPX1 Antibody (3M540) can be used in WB,ICC,IHC,FCM. |
| 交叉反应 | Human |
| 验证活性 | 1. Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution. Positive control: Lane 1: THP-1, Lane 2: HepG2, Lane 3: 293T. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GPX1 antibody. Counter stained with hematoxylin. 3. ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining GPX1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of HepG2 cells with GPX1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000; IHC: 1:200; ICC: 1:200; FCM: 1:100-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead. The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 22 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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