Anti-GHR/Growth Hormone R Polyclonal Antibody is a Rabbit antibody targeting GHR/Growth Hormone R. Anti-GHR/Growth Hormone R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Sheep)

1. Blank control: A549 (blue), the cells were fixed with 2% paraformaldehyde (10 min).
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 5 μL in 100 μL 1X PBS containing 0.5% BSA (green).
2. This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (TMAB-00768) at 1:300 followed by conjugation to a secondary antibody and staining.
3. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GHR Polyclonal Antibody, Unconjugated (TMAB-00768) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Positive control: A549 cells
Concebtration: 1 μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
5. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Testis (Rat) Lysate at 40 μg
Lane 4: Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-Growth hormone receptor (TMAB-00768) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130/110 kDa
Observed band size: 130 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human GHR

Human

Cell differentiation,Growth hormones,Hormones