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Anti-GCLM Antibody (9X353)
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产品编号 TMAC-01635
别名 GSH0_HUMAN, GSC light chain, Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, Glutamate cysteine ligase regulatory subunit, GLCLR, GCS light chain, GCLM, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit, Gamma ECS regulatory subunit
Anti-GCLM Antibody (9X353) 是一种 Rabbit 抗体，靶向 GCLM。Anti-GCLM Antibody (9X353) 可用于 WB,ICC/IF,IHC,FCM,IP。
Anti-GCLM Antibody (9X353)
Anti-GCLM Antibody (9X353)
还可以产品编号 TMAC-01635 别名 GSH0_HUMAN, GSC light chain, Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, Glutamate cysteine ligase regulatory subunit, GLCLR, GCS light chain, GCLM, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit, Gamma ECS regulatory subunit
Anti-GCLM Antibody (9X353) 是一种 Rabbit 抗体，靶向 GCLM。Anti-GCLM Antibody (9X353) 可用于 WB,ICC/IF,IHC,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-GCLM Antibody (9X353) is a Rabbit antibody targeting GCLM. Anti-GCLM Antibody (9X353) can be used in WB,ICC/IF,IHC,FCM,IP. |
| 别名 | GSH0_HUMAN, GSC light chain, Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, Glutamate cysteine ligase regulatory subunit, GLCLR, GCS light chain, GCLM, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit, Gamma ECS regulatory subunit |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of GCLM on different cell lysates using anti-GCLM antibody at 1/500 dilution. Positive control: Lane 1: A431, Lane 2: PC-12, Lane 3: NIH-3T3. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GCLM antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GCLM antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-GCLM antibody. Counter stained with hematoxylin. 5. ICC staining GCLM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining GCLM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining GCLM in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with GCLM antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-100; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Gamma-glutamylcysteine synthetase (γ-GCS) is the rate limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. γ-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human gamma-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human γ-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of γ-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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