Anti-Galectin 3 Antibody (9H353) is a Mouse antibody targeting Galectin 3. Anti-Galectin 3 Antibody (9H353) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

9H353

Human,Rat

1. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (ascites of TMAB-06288) at 1: 1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (ascites of TMAB-06288) at 1: 1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (ascites of TMAB-06288) at 1: 1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (ascites of TMAB-06288) at 1: 1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (ascites of TMAB-06288) at 1: 1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (ascites of TMAB-06288) at 1: 1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (ascites of TMAB-06288) at 1: 1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (ascites of TMAB-06288) at 1: 1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
9. 25 ug total protein per lane of various lysates (see on figure) probed with Galectin 3 monoclonal antibody, unconjugated (TMAB-06288) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
10. 25 ug total protein per lane of various lysates (see on figure) probed with Galectin 3 monoclonal antibody, unconjugated (TMAB-06288) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
11. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galectin 3 Monoclonal Antibody, Unconjugated (TMAB-06288) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, BF488), DAPI (blue) was used to stain the cell nuclei.
12. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galectin 3 Monoclonal Antibody, Unconjugated (TMAB-06288) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, BF488), DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

human Galectin 3 full length protein

Human

LGALS3

Galectin-3

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.