Anti-G6PC Polyclonal Antibody is a Rabbit antibody targeting G6PC. Anti-G6PC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glucose 6 phosphatase alpha) Polyclonal Antibody, Unconjugated (TMAB-00724) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. U-937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TMAB-00724 Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
3. Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human U937cell lysates
Lane 3: Human HL-60 cell lysates
Lane 4: Human HepG2 cell lysates
Lane 5: Human SH-SY5Y cell lysates
Primary: Anti-Glucose 6 phosphatase alpha (TMAB-00724) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 35 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2ug/test

Polyclonal

KLH conjugated synthetic peptide: human Glucose 6 phosphatase alpha

Human

Metabolism of carbohydrates,Other Antibodies,Carbohydrate metabolism,Cancer