Anti-FZD7 Polyclonal Antibody is a Rabbit antibody targeting FZD7. Anti-FZD7 Polyclonal Antibody can be used in FCM,WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,GuineaPig)

1. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Frizzled 7 antibody (TMAB-00723)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Frizzled 7 (TMAB-00723) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
3. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Muscle (Mouse) Lysate at 40 μg
Muscle (Rat) Lysate at 40 μg
Primary: Anti-Frizzled 7 (TMAB-00723) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa

WB: 1:500-2000; FCM: 3ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Frizzled 7

Human

FZD7

Frizzled-7

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.