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顶部生物活性储存和溶解信息计算器

Anti-FUBP1 Antibody (1H129)

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Anti-FUBP1 Antibody (1H129)
产品编号 TMAC-01570
别名 hDH V, FUSE-binding protein 1, FUBP 1, FBP, Far upstream element-binding protein 1, DNA helicase V

Anti-FUBP1 Antibody (1H129) 是一种 Rabbit 抗体，靶向 FUBP1。Anti-FUBP1 Antibody (1H129) 可用于 WB,ICC/IF,IHC,FCM。

Anti-FUBP1 Antibody (1H129)

Anti-FUBP1 Antibody (1H129)

Rating icon 还可以
Anti-FUBP1 Antibody (1H129)
产品编号 TMAC-01570 别名 hDH V, FUSE-binding protein 1, FUBP 1, FBP, Far upstream element-binding protein 1, DNA helicase V

Anti-FUBP1 Antibody (1H129) 是一种 Rabbit 抗体，靶向 FUBP1。Anti-FUBP1 Antibody (1H129) 可用于 WB,ICC/IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-FUBP1 Antibody (1H129) is a Rabbit antibody targeting FUBP1. Anti-FUBP1 Antibody (1H129) can be used in WB,ICC/IF,IHC,FCM.
别名hDH V, FUSE-binding protein 1, FUBP 1, FBP, Far upstream element-binding protein 1, DNA helicase V
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of FUBP1 on different lysates using anti-FUBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Raji.
2. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
7. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
8. ICC staining FUBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. ICC staining FUBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. ICC staining FUBP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
11. Flow cytometric analysis of Jurakt cells with FUBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IHC
FCM
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Activation of FUSE, the far upstream element, is required for the proper ex-pression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP1 (FUSE-binding protein or far upstream element-binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL-60 cells, FBP1 (FBP), FBP2 and FBP3 comprise a family of single-stranded DNA-binding proteins that specifically bind to FUSE elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxyl-terminal activation domains. Expression of FBP1 is detected in undifferentiated cells and is substantially decreased following cellular differentiation.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q96AE4
化学信息
分子量Theoretical: 74 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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