Anti-Fragilis/IFITM3 Antibody (5N399) is a Rabbit antibody targeting Fragilis/IFITM3. Anti-Fragilis/IFITM3 Antibody (5N399) can be used in WB,ICC/IF,IHC,FCM.

IgG

5N399

Human

1. Western blot analysis of Fragilis on different cell lysates using anti-Fragilis antibody at 1/500 dilution. Positive control: Lane 1: Hela, Lane 2: HepG2, Lane 3: Hela.
2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.
4. ICC staining Fragilis in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Fragilis in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Fragilis in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of MCF-7 cells with Fragilis antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

WB: 1:1000-5000; IHC: 1:50-100; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

Unconjugated

Recombinant Protein