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Anti-Flagellin Antibody (7U52)
还可以产品编号 TMAY-02859
Anti-Flagellin Antibody (7U52) 是一种 Mouse 抗体，靶向 Flagellin。Anti-Flagellin Antibody (7U52) 可用于 WB,ELISA。
Anti-Flagellin Antibody (7U52)
Anti-Flagellin Antibody (7U52)
还可以产品编号 TMAY-02859
Anti-Flagellin Antibody (7U52) 是一种 Mouse 抗体，靶向 Flagellin。Anti-Flagellin Antibody (7U52) 可用于 WB,ELISA。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Flagellin Antibody (7U52) is a Mouse antibody targeting Flagellin. Anti-Flagellin Antibody (7U52) can be used in WB,ELISA. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 7U52 |
| 交叉反应 | Listeria monocytogenes |
| 验证活性 | 1. Anti-Listeria monocytogenes flagellin / flaA mouse monoclonal antibody at 1:1000 dilution. -Sample:Listeria monocytogenes flagellin / flaA Recombinant Protein -Lane A: 200ng -Lane B: 50ng -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 2. Anti-Listeria monocytogenes flagellin / flaA mouse monoclonal antibody at 1:1000 dilution. -Sample:Listeria monocytogenes flagellin / flaA Recombinant Protein -Lane A: 10ng -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. |
| 应用 | WB,ELISA |
| 推荐剂量 | WB: 1:1000-1:5000; ELISA: 1:1000-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Listeria monocytogenes Listeria monocytogenes flagellin / FlaA (TMPY-03465; YP_013330.1; Met1-Ser287). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either FlaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the FlaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a FlaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the FlaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Listeria monocytogenes Listeria monocytogenes flagellin / FlaA protein (TMPY-03465) |
| 抗原种属 | Listeria monocytogenes |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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