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顶部产品介绍偶联与修饰储存和溶解信息计算器

Anti-Flag Tag Antibody-Biotin (3S989)

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货号 TMAS-00123B

别名 flagtag, DYKDDDDK-tag

Anti-Flag Tag Antibody-Biotin (3S989) 是一种 Rabbit 抗体，靶向 Flag Tag。Anti-Flag Tag Antibody-Biotin (3S989) 可用于 ELISA, WB。

Anti-Flag Tag Antibody-Biotin (3S989)

Anti-Flag Tag Antibody-Biotin (3S989)

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货号 TMAS-00123B 别名 flagtag, DYKDDDDK-tag

Anti-Flag Tag Antibody-Biotin (3S989) 是一种 Rabbit 抗体，靶向 Flag Tag。Anti-Flag Tag Antibody-Biotin (3S989) 可用于 ELISA, WB。

规格价格库存数量
40 μg
¥ 3,218
5日内发货
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产品介绍

生物活性
产品描述
Anti-Flag Tag Antibody-Biotin (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-Biotin (3S989) can be used in ELISA, WB.
别名flagtag, DYKDDDDK-tag
Ig Type
IgG
克隆号
3S989
特异性
DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins
验证活性
1. Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) binds with DYKDDDDK Tag.
Coating antigen: streptavidin, 5 µg/ml.
Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) dilution start from 1,000 ng/ml.
Secondary Antibody: Mouse Anti-Rabbit IgG Antibody [HRP], mAb.
EC50= 83.47 ng/ml.
2. Sensitivity and specificity of Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) to N-DYKDDDDK-tag Fusion Protein by Western blot.
Lane 1: N-terminal DYKDDDDK-tagged fusion protein (25 ng).
Lane 2: N-terminal DYKDDDDK-tagged fusion protein (10 ng).
Lane 3: N-terminal DYKDDDDK-tagged fusion protein (5 ng).
Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng).
Primary Antibody: Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) 1 μg/ml.
Secondary Antibody: Streptavidin-HRP 0.2 μg/ml.
3. Sensitivity and specificity of Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) to C-DYKDDDDK-tag Fusion Protein by Western blot.
Lane 1: C-terminal DYKDDDDK-tagged fusion protein (25 ng).
Lane 2: C-terminal DYKDDDDK-tagged fusion protein (10 ng).
Lane 3: C-terminal DYKDDDDK-tagged fusion protein (5 ng).
Lane 4: C-terminal DYKDDDDK-tagged fusion protein (1 ng).
Primary Antibody: Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) 1 μg/ml.
Secondary Antibody: Streptavidin-HRP 0.2 μg/ml.
应用ELISAWB
推荐剂量
ELISA: 0.2-0.5 μg/ml; WB: 1 μg/ml
抗体种类
Monoclonal
宿主来源Rabbit
纯化方式Protein A affinity column
性状Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
缓冲液Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
研究背景0
偶联与修饰
偶联
Biotin
其他可选修饰符
Unconjugated/HRP/FITC
可提供抗体种类
4
抗原信息
免疫原
KLH conjugated synthetic peptide: DYKDDDDK
储存&溶解度
储存方式The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储-20°C for 1 year

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