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顶部产品介绍偶联与修饰储存和溶解信息计算器

Anti-Flag Tag Antibody (3S989)

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货号 TMAS-00123

别名 flagtag, DYKDDDDK-tag

Anti-Flag Tag Antibody (3S989) 是一种 Rabbit 抗体，靶向 Flag Tag。Anti-Flag Tag Antibody (3S989) 可用于 ELISA, IP, WB。

Anti-Flag Tag Antibody (3S989)

Anti-Flag Tag Antibody (3S989)

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货号 TMAS-00123 别名 flagtag, DYKDDDDK-tag

Anti-Flag Tag Antibody (3S989) 是一种 Rabbit 抗体，靶向 Flag Tag。Anti-Flag Tag Antibody (3S989) 可用于 ELISA, IP, WB。

规格价格库存数量
40 μg
¥ 3,218
5日内发货
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产品介绍

生物活性
产品描述
Anti-Flag Tag Antibody (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody (3S989) can be used in ELISA, IP, WB.
别名flagtag, DYKDDDDK-tag
Ig Type
IgG
克隆号
3S989
特异性
DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins
验证活性
1. Anti-Flag Tag Antibody (3S989) (TMAS-00123) binds with DYKDDDDK Tag.
Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml.
Anti-Flag Tag Antibody (3S989) (TMAS-00123) dilution start from 1,000 ng/ml.
EC50= 47.78 ng/ml.
2. Sensitivity and specificity of Anti-Flag Tag Antibody (3S989) (TMAS-00123) to DYKDDDDK-tag Fusion Protein by Western blot.
A: N-terminal DYKDDDDK-tagged fusion protein (25 ng, 10 ng, 5 ng)
B: C-terminal DYKDDDDK-tagged fusion protein (25 ng, 10 ng, 5 ng)
C: Internal positions DYKDDDDK-tagged fusion protein (50ng, 25 ng, 10 ng, 5 ng)
Primary Antibody: Anti-Flag Tag Antibody (3S989) (TMAS-00123) 0.2 μg/ml.
Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye800] 0.125 μg/ml.
3. Western blot analysis of immunoprecipitation from cell lysates containing DYKDDDDK fusion protein by using Anti-Flag Tag Antibody (3S989) (TMAS-00123).
Lane 1: Anti-Flag Tag Antibody (3S989) (TMAS-00123) (4 μg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein.
Lane 2: Rabbit IgG (4 μg)+ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein.
Lane 3: N-terminal DYKDDDDK tagged fusion protein (Input).
Lane 4: Anti-Flag Tag Antibody (3S989) (TMAS-00123) (4 μg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein.
Lane 5: Rabbit IgG (4 μg)+ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein.
Lane 6: C-terminal DYKDDDDK tagged fusion protein (Input).
Primary Antibody: Anti-Flag Tag Antibody (3S989) (TMAS-00123) 0.2 μg/ml.
Secondary Antibody:Goat anti-Rabbit IgG (H&L) [IRDye800] 0.125 μg/ml.
应用ELISAIPWB
推荐剂量
ELISA: 0.05-0.2 μg/ml; IP: 0.2-1 μg/ml; WB: 0.2-1 μg/ml
抗体种类
Monoclonal
宿主来源Rabbit
纯化方式Protein A affinity column
性状Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
缓冲液Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
研究背景0
偶联与修饰
偶联
Unconjugated
其他可选修饰符
HRP/Biotin/FITC
可提供抗体种类
4
抗原信息
免疫原
KLH conjugated synthetic peptide: DYKDDDDK
储存&溶解度
储存方式The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储-20°C for 1 year

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