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Anti-ERP72 Antibody (5I583) 是一种 Rabbit 抗体,靶向 ERP72。Anti-ERP72 Antibody (5I583) 可用于 ICC/IF。
Anti-ERP72 Antibody (5I583) 是一种 Rabbit 抗体,靶向 ERP72。Anti-ERP72 Antibody (5I583) 可用于 ICC/IF。
产品描述 | Anti-ERP72 Antibody (5I583) is a Rabbit antibody targeting ERP72. Anti-ERP72 Antibody (5I583) can be used in ICC/IF. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 5I583 |
交叉反应 | Human |
验证活性 | Immunofluorescence staining of Human PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PDIA4 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. |
应用 | ICC/IF |
推荐剂量 | ICC-IF: 1:20-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human ERP72 / PDIA4 (rh ERP72 / PDIA4; TMPY-03178; P13667; Met1-Thr641). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | ERP72, also known as PDIA4, is an endoplasmic reticulum luminal protein which belongs to the protein disulfide isomerase family. ERP72 is a stress protein and participates in the catalysis of protein-S-S-bond rearrangement. Both PDIA4 and PDIA3 function as proteases, protein disulfide isomerases, phospholipases or an arrangement of these. ERP72 compose part of a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. |
偶联 | Unconjugated |
免疫原 | Recombinant Human ERP72 / PDIA4 Protein (TMPY-03178) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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