Anti-ER beta Polyclonal Antibody is a Rabbit antibody targeting ER beta. Anti-ER beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.

IgG

Human,Mouse,Rat

1. Sample:
Kidney (Mouse) lysate at 30 μg;
Brain (Rat) lysate at 30 μg;
Primary: Anti-ER-beta (TMAB-00632) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000;
Predicted band size: 58 kDa
Observed band size: 58 kDa
2. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: Testis (Rat) Lysate at 40 μg
Lane 3: Ovary (Mouse) Lysate at 40 μg
Lane 4: Uterus (Mouse) Lysate at 40 μg
Lane 5: Uterus (Rat) Lysate at 40 μg
Primary: Anti-Estrogen receptor beta (TMAB-00632) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58-62 kDa
3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Cerebellum tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Cerebellum tissue lysates
Lane 7: Human MCF-7 cell lysates
Lane 8: Human Jurkat cell lysates
Lane 9: Human U-2 OS cell lysates
Lane 10: Human A431 cell lysates
Primary: Anti-ESR2 (TMAB-00632) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 63 kDa
14. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Estrogen Receptor beta) polyclonal Antibody, unconjugated (TMAB-00632) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; ICC/IF: 1:50-200; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human ER-beta

Human

Estrogen,Receptors,ChIP kits,Estrogen,Zinc Finger,Gonadotrophic axis,Estrogen